GP+envAM-12

 
Species source: mouse
Organ source: embryo; amphotropic retroviral packaging line
Plasmid:
  • pLXSN
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Gentamicin - 1mg/ml - 3weeks
Reference:
  • D. S. Min, T. K. Kwon, W. S. Park, J. S. Chang, S. K. Park, B. H. Ahn, Z. Y. Ryoo, Y. H. Lee, Y. S. Lee and D. J. Rhie 2001. Neoplastic transformation and tumorigenesis associated with overexpression of phospholipase D isozymes in cultured murine fibroblasts