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Plasmid/Vector
Antibiotics
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CHO-K1
CHO-K1
Species source:
hamster, Chinese
Organ source:
ovary
Plasmid:
pCMV-Insig-2-Myc
Culture/Media:
DMEM + F12 medium
Temperature:
37
Atmosphere:
8-9% CO2
Antibiotics:
Geneticin (G418) - 700μg/ml -
Reference:
D. Yabe, M. S. Brown and J. L. Goldstein 2002. Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins