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H9 hES
H9 hES
Plasmid:
pEB-IRES
pEBneo
pEB-SV40polyA
Culture/Media:
DMEM + F12 medium
Temperature:
37
Atmosphere:
5% CO2
Antibiotics:
Geneticin (G418) - 200μg/ml - 2days
Reference:
C. P. REN, M. Zhao, W. J. SHAN, X. Y. YANG, Z. H. YIN, X. J. JIANG, H. B. ZHANG and K. T. YAO 2005. Establishment of Human Embryonic Stem Cell Line Stably Expressing Epstein Barr Virus Encoded Nuclear Antigen 1