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Cell-Lines
Plasmid/Vector
Antibiotics
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CHO-K1
CHO-K1
Species source:
hamster, Chinese
Organ source:
ovary
Plasmid:
pVSVG-T7
Culture/Media:
DMEM + F12 medium
Temperature:
37
Atmosphere:
8-9% CO2
Antibiotics:
Geneticin (G418) - 700μg/ml -
Reference:
A. Nohturfft, D. Yabe, J. L. Goldstein, M. S. Brown and P. J. Espenshade 2000. Regulated step in cholesterol feedback localized to budding of SCAP from ER membranes