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Plasmid/Vector
Antibiotics
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hESCs
hESCs
Plasmid:
pTP6
Culture/Media:
RPMI 1640 Medium
Antibiotics:
Hygromycin - 1μg/ml - 10days
Reference:
X. Zhang, I. Neganova, S. Przyborski, C. Yang, M. Cooke, S. P. Atkinson, G. Anyfantis, S. Fenyk, W. N. Keith and S. F. Hoare 2009. A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A