MCF7

 
Species source: human
Organ source: pleural effusion (metastasis); adenocarcinoma (mammary gland primary)
Plasmid:
  • pEF-BOS
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 600μg/ml -
Reference:
  • A. D. Gonzalez, M. Kaya, W. Shi, H. Song, J. R. Testa, L. Z. Penn and J. Filmus 1998. OCI-5/GPC3, a glypican encoded by a gene that is mutated in the Simpson-Golabi-Behmel overgrowth syndrome, induces apoptosis in a cell line–specific manner