T lymphocyte; ZAP-70 negative; model for T cell receptor signaling
Plasmid:
pcDNA3-ZAP
pBJ-mZAPKD
Antibiotics:
Geneticin (G418) - 2mg/ml -
Reference:
B. L. Williams, K. L. Schreiber, W. Zhang, R. L. Wange, L. E. Samelson, P. J. Leibson and R. T. Abraham 1998. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T-cell line