Lec2

 
Species source: hamster, Chinese
Organ source: ovary; reduced transport of CMP-sialic acid into Golgi compartment
Plasmid:
  • pcDNA3
Culture/Media: α-modified minimal essential medium
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 400μg/ml -
Reference:
  • G. Wei, X. Bai, A. K. Sarkar and J. D. Esko 1999. Formation of HNK-1 determinants and the glycosaminoglycan tetrasaccharide linkage region by UDP-GlcUA: galactose 1, 3-glucuronosyltransferases