U-2 OS

 
Species source: human
Organ source: bone; osteosarcoma
Plasmid:
  • pcDNA3–GFPL
  • pcDNA3–GFP–let-7 (PE)
  • pcDNA3–GFP–miR-21 (8 × BU)
Culture/Media: Dulbecco's modified Eagle's medium
Antibiotics:
  • Geneticin (G418) - 2000μg/ml - 14d
Reference:
  • H. H. Qi, P. P. Ongusaha, J. Myllyharju, D. Cheng, O. Pakkanen, Y. Shi, S. W. Lee and J. Peng 2008. Prolyl 4-hydroxylation regulates Argonaute 2 stability