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Plasmid/Vector
Antibiotics
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U-2 OS
U-2 OS
Species source:
human
Organ source:
bone; osteosarcoma
Plasmid:
pcDNA3–GFPL
pcDNA3–GFP–let-7 (PE)
pcDNA3–GFP–miR-21 (8 × BU)
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Geneticin (G418) - 2000μg/ml - 14d
Reference:
H. H. Qi, P. P. Ongusaha, J. Myllyharju, D. Cheng, O. Pakkanen, Y. Shi, S. W. Lee and J. Peng 2008. Prolyl 4-hydroxylation regulates Argonaute 2 stability