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Antibiotics
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PC12-27
PC12-27
Plasmid:
pREP4
Culture/Media:
Dulbecco's modified Eagle's medium
Atmosphere:
10% CO2
Antibiotics:
Hygromycin B - 450μg/ml - 15days
Reference:
N. Corradi, B. Borgonovo, E. Clementi, M. Bassetti, G. Racchetti, G. G. Consalez, W. B. Huttner, J. Meldolesi and P. Rosa 1996. Overall lack of regulated secretion in a PC12 variant cell clone