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Plasmid/Vector
Antibiotics
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PC12
PC12
Plasmid:
pMEK
Culture/Media:
Dulbecco's modified Eagle's medium
Temperature:
37
Atmosphere:
5% CO2
Antibiotics:
Geneticin (G418) - 500μg/ml - 28-42days
Reference:
Z. Wang, T. J. Dillon, V. Pokala, S. Mishra, K. Labudda, B. Hunter and P. J. S. Stork 2006. Rap1-mediated activation of extracellular signal-regulated kinases by cyclic AMP is dependent on the mode of Rap1 activation