MDCK (NBL-2)

 
Species source: dog
Organ source: kidney
Plasmid:
  • pRc/CMV1
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 400μg/ml - 14-21days
Reference:
  • Y. Doyon, W. Home, P. Daull and D. LeBel 2002. Effect of C-domain N-glycosylation and deletion on rat pancreatic alpha-amylase secretion and activity