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Cell-Lines
Plasmid/Vector
Antibiotics
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GLUTag
GLUTag
Plasmid:
pcDNA3.1(+)
Culture/Media:
Dulbecco's modified Eagle's medium
Temperature:
37
Atmosphere:
5% CO2
Antibiotics:
Geneticin (G418) - 200μg/ml - 2days
Reference:
H. Bara and A. Sambanis 2008. Insulin-secreting L-cells for the treatment of insulin-dependent diabetes