CD40-B cells | Cell-culture Database

Cell-Lines
Plasmid/Vector
Antibiotics

CD40-B

Plasmid:	pcDNA3.1
Culture/Media:	Iscove's modified Dulbecco's medium
Temperature:	37
Atmosphere:	5% CO2
Antibiotics:	Puromycin - 0.6μg/ml - 2days
Reference:	E. Kondo, M. S. Topp, H. P. Kiem, Y. Obata, Y. Morishima, K. Kuzushima, M. Tanimoto, M. Harada, T. Takahashi and Y. Akatsuka 2002. Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells

This project is supported byTOKU-Ewhich specializes in manufacturing ultra-pure antibiotics for a broad spectrum of biotechnology applications as well as for the pharmaceutical industry. TOKU-E is a global leader in biotechnology innovation, offering great benefits and applications to the biopharmaceutical and diagnostic industries as well as for biotechnology research communities.
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