Hep3B

 
Plasmid:
  • pcDNA3
Culture/Media: Minimal essential with Hank's salts
Temperature: 37
Atmosphere: 5% CO2 and 95% air
Antibiotics:
  • Geneticin (G418) - 1000μg/ml - 21days
Reference:
  • G. X. Wu, Y. M. Lin, T. H. Zhou, H. Gao and G. Pei 2000. Significant down-regulation of alpha-albumin in human hepatoma and its implication