BT-474

 
Species source: human
Organ source: mammary gland; ductal carcinoma
Plasmid:
  • pcDNA3.1/Hyg(+)
  • pCEP4
  • pcDNA3
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Hygromycin B - 200μg/ml - 14-21days
  • Geneticin (G418) - 700μg/ml - 14-21days
  • Puromycin - 1μg/ml - 14-21days
Reference:
  • J. H. Lin, C. H. Tsai, J. S. Chu, J. Y. Chen, K. Takada and J. Y. Shew 2007. Dysregulation of HER2/HER3 signaling axis in Epstein-Barr virus-infected breast carcinoma cells