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Cell-Lines
Plasmid/Vector
Antibiotics
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SD1
SD1
Plasmid:
pTET-OFF
pTRE
pcDNA3 Zeo+
Culture/Media:
RPMI 1640 Medium
Antibiotics:
Zeocin - 700μg/ml -
Geneticin (G418) - 1000μg/ml -
Hygromycin B - 400μg/ml -
Doxycycline (Doxycycline hyclate) - 50μg/ml -
Reference:
S. Yamashiro, Y. Yamakita, S. Ono and F. Matsumura 1998. Fascin, an actin-bundling protein, induces membrane protrusions and increases cell motility of epithelial cells