ES cell

 
Plasmid:
  • Mrad9neo/loxP
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 200μg/ml -
Reference:
  • P. Roos-Mattjus, K. M. Hopkins, A. J. Oestreich, B. T. Vroman, K. L. Johnson, S. Naylor, H. B. Lieberman and L. M. Karnitz 2003. Phosphorylation of human Rad9 is required for genotoxin-activated checkpoint signaling