1180MEL

 
Plasmid:
  • pcDNA3/neo
Culture/Media: RPMI 1640 Medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 1000μg/ml - 2-3weeks
Reference:
  • C. C. Chang, M. Campoli, N. P. Restifo, X. Wang and S. Ferrone 2005. Immune selection of hot-spot 2-microglobulin gene mutations, HLA-A2 allospecificity loss, and antigen-processing machinery component down-regulation in melanoma cells derived from recurrent metastases following immunotherapy