1180MEL

Cell-Lines
Plasmid/Vector
Antibiotics

Plasmid:	pcDNA3/neo
Culture/Media:	RPMI 1640 Medium
Temperature:	37
Atmosphere:	5% CO2
Antibiotics:	Geneticin (G418) - 1000μg/ml - 2-3weeks
Reference:	C. C. Chang, M. Campoli, N. P. Restifo, X. Wang and S. Ferrone 2005. Immune selection of hot-spot 2-microglobulin gene mutations, HLA-A2 allospecificity loss, and antigen-processing machinery component down-regulation in melanoma cells derived from recurrent metastases following immunotherapy

This project is supported byTOKU-Ewhich specializes in manufacturing ultra-pure antibiotics for a broad spectrum of biotechnology applications as well as for the pharmaceutical industry. TOKU-E is a global leader in biotechnology innovation, offering great benefits and applications to the biopharmaceutical and diagnostic industries as well as for biotechnology research communities.
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