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Cell-Lines
Plasmid/Vector
Antibiotics
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rHSCs
rHSCs
Plasmid:
pcDNA3
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Geneticin (G418) - 400μg/ml - 2-3weeks
Reference:
W. B. Liu, C. Q. Yang, W. Jiang, Y. Q. Wang, J. S. Guo, B. M. He and J. Y. Wang 2003. Inhibition on the production of collagen type I, III of activated hepatic stellate cells by antisense TIMP-1 recombinant plasmid