3T3-L1

 
Species source: mouse
Organ source: embryo
Plasmid:
  • pRNAT-U6.2/Lenti
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5%CO2
Antibiotics:
  • Geneticin (G418) - 400μg/ml -
Reference:
  • Y. Liu, F. Park, J. L. Pietrusz, G. Jia, R. J. Singh, B. C. Netzel and M. Liang 2008. Suppression of 11 -hydroxysteroid dehydrogenase type 1 with RNA interference substantially attenuates 3T3-L1 adipogenesis