HeLa

 
Species source: human
Organ source: cervix; adenocarcinoma
Plasmid:
  • pMiNeo
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 600μg/ml -
Reference:
  • J. Sampath, P. R. Long, R. L. Shepard, X. Xia, V. Devanarayan, G. E. Sandusky, W. L. Perry III, A. H. Dantzig, M. Williamson and M. Rolfe 2003. Human SPF45, a splicing factor, has limited expression in normal tissues, is overexpressed in many tumors, and can confer a multidrug-resistant phenotype to cells