NIH/3T3

 
Species source: mouse
Organ source: embryo
Plasmid:
  • pIND-V12-H-Ras
  • pIND-V12-Rac1
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 400μg/ml - 5weeks
Reference:
  • H. J. Cho, H. G. Jeong, J. S. Lee, E. R. Woo, J. W. Hyun, M. H. Chung and H. J. You 2002. Oncogenic H-Ras enhances DNA repair through the ras/phosphatidylinositol 3-kinase/rac1 pathway in NIH3T3 cells