BHK-21 (C-13)

 
Species source: hamster, Syrian golden
Organ source: kidney
Plasmid:
  • pSFV1-d1EGFP
Culture/Media: GMEM medium
Temperature: 28
Atmosphere: 5% CO2
Antibiotics:
  • Puromycin - 10μg/ml -
Reference:
  • K. Tamm, A. Merits and I. Sarand 2008. Mutations in the nuclear localization signal of nsP2 influencing RNA synthesis, protein expression and cytotoxicity of Semliki Forest virus