MCF7

 
Species source: human
Organ source: pleural effusion (metastasis); adenocarcinoma (mammary gland primary)
Plasmid:
  • pSV2neo
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 800μg/ml -
Reference:
  • J. R. MacDougall and L. M. Matrisian 2000. Targets of extinction: identification of genes whose expression is repressed as a consequence of somatic fusion between cells representing basal and luminal mammary epithelial phenotypes