CHO-K1

 
Species source: hamster, Chinese
Organ source: ovary
Plasmid:
  • pCMV-Insig-2-Myc
Culture/Media: DMEM + F12 medium
Temperature: 37
Atmosphere: 8-9% CO2
Antibiotics:
  • Geneticin (G418) - 700μg/ml -
Reference:
  • D. Yabe, M. S. Brown and J. L. Goldstein 2002. Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins