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Cell-Lines
Plasmid/Vector
Antibiotics
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BJAB
BJAB
Plasmid:
pcDNA3
pcDNA3-FLAG
pcDNA3-Myc
Culture/Media:
RPMI 1640 Medium
Antibiotics:
Geneticin (G418) - 4000μg/ml - 48h
Hygromycin - 1000μg/ml - 48h
Reference:
A. Krueger, I. Schmitz, S. Baumann, P. H. Krammer and S. Kirchhoff 2001. Cellular FLICE-inhibitory protein splice variants inhibit different steps of caspase-8 activation at the CD95 death-inducing signaling complex