hESCs

 
Plasmid:
  • pTP6
  • pTP6-Lefty2
  • pTP6-Cerb-S
Culture/Media: KO-DMEM
Antibiotics:
  • Hygromycin - 1μg/ml - 3days
Reference:
  • J. R. Smith, L. Vallier, G. Lupo, M. Alexander, W. A. Harris and R. A. Pedersen 2008. Inhibition of Activin/Nodal signaling promotes specification of human embryonic stem cells into neuroectoderm