293

 
Species source: human
Organ source: kidney; fetal
Plasmid:
  • pcDNA6.2/GW/D-TOPO
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 800μg/ml -
  • Blasticidin - 20μg/ml -
Reference:
  • P. K. Lawrence, R. P. Dassanayake, D. P. Knowles and S. Srikumaran 2007. Transfection of non-susceptible cells with Ovis aries recombinant lymphocyte function-associated antigen 1 renders susceptibility to Mannheimia haemolytica leukotoxin