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Plasmid/Vector
Antibiotics
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HEK
HEK
Plasmid:
pIRES–T7POL
Culture/Media:
Dulbecco's modified Eagle's medium
Temperature:
37
Atmosphere:
5%CO2
Antibiotics:
Geneticin (G418) - 500μg/ml - 28d
Reference:
T. H. Wang, S. H. Yu and L. C. Au 2008. Facilitated in vivo synthesis of ribonucleic acid and protein via T7 RNA polymerase