HeLa

 
Species source: human
Organ source: cervix; adenocarcinoma
Plasmid:
  • pIRES–T7POL
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5%CO2
Antibiotics:
  • Geneticin (G418) - 500μg/ml - 28d
Reference:
  • T. H. Wang, S. H. Yu and L. C. Au 2008. Facilitated in vivo synthesis of ribonucleic acid and protein via T7 RNA polymerase