HeLa

 
Species source: human
Organ source: cervix; adenocarcinoma
Plasmid:
  • pREP4.tTA
  • pREP9.nef
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Hygromycin B - 400μg/ml -
  • Geneticin (G418) - 500μg/ml -
  • Tetracycline - 1μg/ml -
Reference:
  • S. J. Cooke, K. Coates, C. H. Barton, T. E. Biggs, S. J. Barrett, A. Cochrane, K. Oliver, J. A. McKeating, M. Harris and D. A. Mann 1997. Regulated expression vectors demonstrate cell-type-specific sensitivity to human immunodeficiency virus type 1 Nef-induced cytostasis