HeLa

 
Species source: human
Organ source: cervix; adenocarcinoma
Plasmid:
  • pSTLN1
  • pcDNA3.1HisLac
Culture/Media: Minimal essential medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 800μg/ml - 6-8weeks
Reference:
  • S. R. Tipnis, D. G. Blake, A. G. Shepherd and L. I. McLellan 1999. Overexpression of the regulatory subunit of gamma-glutamylcysteine synthetase in HeLa cells increases gamma-glutamylcysteine synthetase activity and confers drug resistance