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U-2 OS

Species source:	human
Organ source:	bone; osteosarcoma
Plasmid:	pcDNA3–GFPL pcDNA3–GFP–let-7 (PE) pcDNA3–GFP–miR-21 (8 × BU)
Culture/Media:	Dulbecco's modified Eagle's medium
Antibiotics:	Geneticin (G418) - 2000μg/ml - 14d
Reference:	H. H. Qi, P. P. Ongusaha, J. Myllyharju, D. Cheng, O. Pakkanen, Y. Shi, S. W. Lee and J. Peng 2008. Prolyl 4-hydroxylation regulates Argonaute 2 stability

This project is supported byTOKU-Ewhich specializes in manufacturing ultra-pure antibiotics for a broad spectrum of biotechnology applications as well as for the pharmaceutical industry. TOKU-E is a global leader in biotechnology innovation, offering great benefits and applications to the biopharmaceutical and diagnostic industries as well as for biotechnology research communities.
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