PC12

 
Plasmid:
  • pMEK
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 500μg/ml - 28-42days
Reference:
  • Z. Wang, T. J. Dillon, V. Pokala, S. Mishra, K. Labudda, B. Hunter and P. J. S. Stork 2006. Rap1-mediated activation of extracellular signal-regulated kinases by cyclic AMP is dependent on the mode of Rap1 activation