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Antibiotics
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CD40-B
CD40-B
Plasmid:
pcDNA3.1
Culture/Media:
Iscove's modified Dulbecco's medium
Temperature:
37
Atmosphere:
5% CO2
Antibiotics:
Puromycin - 0.6μg/ml - 2days
Reference:
E. Kondo, M. S. Topp, H. P. Kiem, Y. Obata, Y. Morishima, K. Kuzushima, M. Tanimoto, M. Harada, T. Takahashi and Y. Akatsuka 2002. Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells