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Antibiotics
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Hep3B
Hep3B
Plasmid:
pcDNA3
Culture/Media:
Minimal essential with Hank's salts
Temperature:
37
Atmosphere:
5% CO2 and 95% air
Antibiotics:
Geneticin (G418) - 1000μg/ml - 21days
Reference:
G. X. Wu, Y. M. Lin, T. H. Zhou, H. Gao and G. Pei 2000. Significant down-regulation of alpha-albumin in human hepatoma and its implication