HeLa Tet-Off

 
Plasmid:
  • pUHGmFH
  • pUHGmFL
  • pHygSRalpha
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Hygromycin - 200μg/ml -
Reference:
  •  0.