SD1

 
Plasmid:
  • pTET-OFF
  • pTRE
  • pcDNA3 Zeo+
Antibiotics:
  • Zeocin - 700μg/ml -
  • Geneticin (G418) - 1000μg/ml -
  • Hygromycin B - 400μg/ml -
  • Doxycycline (Doxycycline hyclate) - 50μg/ml -
Reference:
  • B. Manoury, D. Mazzeo, L. Fugger, N. Viner, M. Ponsford, H. Streeter, G. Mazza, D. C. Wraith and C. Watts 2002. Destructive processing by asparagine endopeptidase limits presentation of a dominant T cell epitope in MBP