SD1

 
Plasmid:
  • pTET-OFF
  • pTRE
  • pcDNA3 Zeo+
Culture/Media: RPMI 1640 Medium
Antibiotics:
  • Zeocin - 700μg/ml -
  • Geneticin (G418) - 1000μg/ml -
  • Hygromycin B - 400μg/ml -
  • Doxycycline (Doxycycline hyclate) - 50μg/ml -
Reference:
  • S. Yamashiro, Y. Yamakita, S. Ono and F. Matsumura 1998. Fascin, an actin-bundling protein, induces membrane protrusions and increases cell motility of epithelial cells