HCT 116

 
Species source: human
Organ source: colon; colorectal carcinoma
Plasmid:
  • pUHD172-1neo
  • pCMV
  • pUHD
Culture/Media: DMEM / F 12 medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • Geneticin (G418) - 800μg/ml - 2weeks
Reference:
  • C. Bossenmeyer-Pourié, R. Kannan, S. Ribieras, C. Wendling, I. Stoll, L. Thim, C. Tomasetto and M. C. Rio 2002. The trefoil factor 1 participates in gastrointestinal cell differentiation by delaying G1-S phase transition and reducing apoptosis