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Plasmid/Vector
Antibiotics
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HeLa Tet-On
HeLa Tet-On
Plasmid:
pTRE
pTK-Hyg
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Geneticin (G418) - 100μg/ml -
Hygromycin - 200μg/ml -
Reference:
L. Pellizzoni, J. Baccon, J. Rappsilber, M. Mann and G. Dreyfuss 2002. Purification of native survival of motor neurons complexes and identification of Gemin6 as a novel component