NIH/3T3

 
Species source: mouse
Organ source: embryo
Plasmid:
  • PRLR
  • pME18S
  • pSV2neo
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Antibiotics:
  • Geneticin (G418) - 400μg/ml - 14d
Reference:
  • R. Nanbu-Wakao, Y. Fujitani, Y. Masuho, M. Muramatu and H. Wakao 2000. Prolactin enhances CCAAT enhancer-binding protein- (C/EBP ) and peroxisome proliferator-activated receptor (PPAR ) messenger RNA expression and stimulates adipogenic conversion of NIH-3T3 cells