NIH/3T3

 
Species source: mouse
Organ source: embryo
Plasmid:
  • WZLhygro
  • WZLhygroPPARγ2
Culture/Media: Dulbecco's modified Eagle's medium
Antibiotics:
  • Hygromycin B - 400μg/ml - 7d
Reference:
  • G. Adelmant, J. D. Gilbert and S. O. Freytag 1998. Human translocation liposarcoma-CCAAT/enhancer binding protein (C/EBP) homologous protein (TLS-CHOP) oncoprotein prevents adipocyte differentiation by directly interfering with C/EBP function