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Antibiotics
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Rat-1/Wnt-1
Rat-1/Wnt-1
Plasmid:
pcDNA3-flag-DN-Tcf-4
pBABE-puro
Culture/Media:
Dulbecco's modified Eagle's medium
Temperature:
37
Atmosphere:
5% CO2
Antibiotics:
Puromycin - 1.5μg/ml - 2weeks
Reference:
0.