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Cell-Lines
Plasmid/Vector
Antibiotics
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CHO
CHO
Species source:
hamster, Chinese
Organ source:
ovary
Plasmid:
hVPAC1-R
hVPAC2-R
rVPAC1-R
rVPAC2-R
Culture/Media:
Ham’s F-12 nutrient mixture
Antibiotics:
Geneticin (G418) - 800μg/ml - 2weeks
Reference:
T. Ito, H. Igarashi, T. K. Pradhan, W. Hou, S. A. Mantey, J. E. Taylor, W. A. Murphy, D. H. Coy and R. T. Jensen 2001. GI side-effects of a possible therapeutic GRF analogue in monkeys are likely due to VIP receptor agonist activity