3T3

 
Species source: mouse
Organ source: embryonic fibroblast
Plasmid:
  • pcDNA3
Culture/Media: Dulbecco's modified Eagle's medium
Temperature: 37
Atmosphere: 5% CO2
Antibiotics:
  • G418 sulfate - 800μg/ml - 10days
Reference:
  • M. Schumann, T. Nakagawa, S. A. Mantey, K. Tokita, D. J. Venzon, S. J. Hocart, R. V. Benya and R. T. Jensen 2003. Importance of amino acids of the central portion of the second intracellular loop of the gastrin-releasing peptide receptor for phospholipase C activation, internalization, and chronic down-regulation