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Plasmid/Vector
Antibiotics
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Species source:
opossum
Organ source:
kidney (cortex); proximal tubule
Plasmid:
pCMV
Culture/Media:
DMEM + F12 medium
Atmosphere:
5% CO2
Antibiotics:
Geneticin (G418) - 500μg/ml -
Reference:
H. Hayashi, K. Szászi, N. Coady-Osberg, W. Furuya, A. P. Bretscher, J. Orlowski and S. Grinstein 2004. Inhibition and redistribution of NHE3, the apical Na+/H+ exchanger, by Clostridium difficile toxin B