Bing

 
Species source: human
Organ source: kidney; amphotropic retroviral packaging line
Plasmid:
  • pWZL-CD8
  • pWZL-Hygro
  • pLPC
Culture/Media: Dulbecco's modified Eagle's medium
Antibiotics:
  • Puromycin - 2μg/ml -
  • Hygromycin - 75μg/ml -
  • Geneticin (G418) - 200μg/ml -
Reference:
  • M. Serrano, A. W. Lin, M. E. McCurrach, D. Beach and S. W. Lowe 1997. Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a