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AtT20
AtT20
Plasmid:
pcDNA3
Culture/Media:
Dulbecco's modified Eagle's medium
Antibiotics:
Geneticin (G418) - 400μg/ml - 2days
Reference:
N. Brakch, F. Allemandou, C. Cavadas, E. Grouzmann and H. R. Brunner 2002. Dibasic cleavage site is required for sorting to the regulated secretory pathway for both pro and neuropeptide Y